Autophagy Contributes to the Maintenance of Genomic Integrity by Reducing Oxidative Stress

Figure 4

Induction of autophagy reduces ROS and micronuclei in senescent cells. (a) The percentage of senescent MSCs at passages 10 (P10) and 18 (P18). Cellular senescence was tested by SA-β-gal staining. (b) Rapa partially restores the autophagic flux in senescent cells. Young and senescent MSCs were treated with Rapa (20 nM) for 24 h for examination of autophagy efficiency (LC3 and p62) using Western blot analysis (the numbers below indicate ratios of LC3-II/LC3-I). (c) Rapa reduces micronuclei in senescent MSCs. Normal and senescent MSCs were treated with Rapa (20 nM) for 24 h; the frequencies of MN were examined at 48 h after Rapa was washed out. (d) Rapa reduces the ROS level of senescent cells. Normal and senescent MSCs were treated with Rapa (20 nM) for 24 h; cellular ROS level was measured by flow cytometry. Each experiment was repeated three times. , , and . SA-β-gal: senescence-associated β-galactosidase.

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