Mitogen- and Stress-Activated Protein Kinase 1 Mediates Alcohol-Upregulated Transcription of Brf1 and tRNA Genes to Cause Phenotypic Alteration

Figure 6

Inhibiting MSK1 pathway caused cellular phenotypic alteration. (a) HepG2-ADH cells were seeded into 6-well plats and grown with different amounts of SB-747651A and 50 mM ethanol. The concentrations of SB-747651A were 0, 2.5, 5, 10, and 20 μM in turn. The pictures were taken under a microscope (Nikon Eclipse TE300). Original magnification ×100. (b, c) The viability and total cell numbers were counted after plated cells. The cells were treated serially by SB-747651A as a dose curve (b) and 50 mM ethanol as indicated. The cells were treated with 10 μM SB-747651A and 50 mM for 1-6 days as a time curve (c). (d, e) The cells were treated as above, and the rates of cell growth were detected by MTT assay. The bars represent of at least three independent determinations. and .

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