Oxidative Medicine and Cellular Longevity / 2020 / Article / Fig 9

Research Article

Identification of Potential Inhibitors of Calcium/Calmodulin-Dependent Protein Kinase IV from Bioactive Phytoconstituents

Figure 9

ATPase inhibition assay of CaMKIV with EA and quercetin. (a) Dose-response curve showing the effect of increasing concentrations of EA (0 to 100 μM) on the kinase activity of CaMKIV. The standard phosphate curve was used to calculate the amount of inorganic phosphate released from ATP in a reaction catalyzed by CaMKIV. (b) Plot depicting the amount of % inhibition in ATPase activity as a function of . The data were fit to estimate the value of IC50 for EA using GraphPad Prism 5.0. (c) The dose-response curve showing the effect of increasing concentrations of quercetin (0 to 100 μM) on the kinase activity of CaMKIV. (d) Plot depicting the amount of % inhibition in ATPase activity as a function of . Results shown here are depicted as of three independent experiments.
(a)
(b)
(c)
(d)

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.