Circadian Clock and OxInflammation: Functional Crosstalk in Cutaneous Homeostasis

Figure 2

Immunofluorescence for NRF2 and NF-κB in dex-synchronized HaCaT cells and arrhythmic ones chronically inflamed with LPS 1 μg/ml and exposed to O₃. Representative pictures of three different experiments are presented. Nuclei (blue) were stained with DAPI. Magnification 40x,  μm. Samples were harvested at different time points (0, 2, and 6 hours after ozone exposure). Quantification of fluorescence in total HaCaT cells (b, d), cytoplasm, and nucleus fractions (e, f) of NRF2 and NF-κB proteins is shown. Data are expressed as from three independent experiments. .

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