Circadian Clock and OxInflammation: Functional Crosstalk in Cutaneous Homeostasis

Figure 4

Relative expression (in percentage) of Ho1 (a) and Nqo1 (b) mRNA in dex-synchronized HaCaT cells and arrhythmic ones chronically inflamed with LPS 1 μg/ml and exposed to O₃ was determined using qRT-PCR. Samples were harvested at different time points (0, 2, and 6 hours after ozone exposure). For all genes, the constitutively expressed 18S rRNA was used to normalization. For each time point, the of three independent experiments is shown. .

(a)

(b)