Oxidative Medicine and Cellular Longevity / 2020 / Article / Fig 4

Research Article

Honokiol Alleviates Methionine-Choline Deficient Diet-Induced Hepatic Steatosis and Oxidative Stress in C57BL/6 Mice by Regulating CFLAR-JNK Pathway

Figure 4

Effects of HNK on the cellular content of TG, ROS, and 2-NBDG and the protein expression of CFLAR, pJNK/JNK, NRF2, and pIRS1/IRS1 in OA- and PA-pretreated NCTC-1469 cells. NCTC-1469 cells were pretreated with OA and PA for 24 h, then treated with HNK for 24 h to detect the cellular viability (a) by MTT method, to detect the contents of TG (b), ROS (c), and 2-NBDG (d), and by the commercial kits, to detect the protein expression of CFLAR, NRF2, pJNK/JNK, and pIRS1/IRS1 (e)–(i) by Western blotting. . # and ##vs. the control group; and vs. the model group.

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