Oxidative Medicine and Cellular Longevity / 2020 / Article / Fig 5

Research Article

Honokiol Alleviates Methionine-Choline Deficient Diet-Induced Hepatic Steatosis and Oxidative Stress in C57BL/6 Mice by Regulating CFLAR-JNK Pathway

Figure 5

Effects of HNK on the expression of CFLAR-JNK pathway-related genes in NCTC-1469 cells transfected with adenovirus-mediated knockdown of Cflar. NCTC-1469 cells were transfected by Ad-shCflar for 24 h, then treated with HNK for 24 h to detect the mRNA and protein expression of CFLAR (a, b), as well as the protein expression of pJNK/JNK, NRF2, and pIRS1/IRS1 (c–f). The mRNA and protein expression was analyzed by RT-qPCR and Western blotting, respectively. . # and ##vs. the control group; and vs. the model group.
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