Withaferin A Exerts Preventive Effect on Liver Fibrosis through Oxidative Stress Inhibition in a Sirtuin 3-Dependent Manner

Figure 2

Effects of WFA on cell viability and toxicity of JS1 and AML12 cells. JS1 cells were treated with DMSO (0.02%, /), PDGF-BB (20 ng/ml), and the indicated concentrations of WFA for 24 h. (a) The effect of WFA on cell viability of activated JS1 cells by an MTS reagent. (b) The toxicity of WFA on activated JS1 cells was examined by a LDH activity assay kit. (c) The toxicity of WFA on inactivated JS1 cells was examined by a LDH activity assay kit. (d) AML12 cells were treated with DMSO (0.02%, /) and the indicated concentrations of APAP and WFA for 24 h. For the statistics of each panel in this figure, data are expressed as (). , , and .

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