Research Article
Withaferin A Exerts Preventive Effect on Liver Fibrosis through Oxidative Stress Inhibition in a Sirtuin 3-Dependent Manner
Figure 7
Effect of WFA on the CCl4-induced collagen production, liver fibrosis, and SIRT3 expression in mice. (a) Liver sections were stained with Masson’s trichrome and picrosirius red reagents, and representative photographs are shown (scale bar: 100 μm), respectively. (b) Measurement of hydroxyproline levels in liver homogenate, . (c) Real-time PCR analyses of SIRT3, α-SMA, fibronectin, and α1(I) procollagen in liver tissues. GAPDH was used as the invariant control. (d) Western blot analyses of liver proteins with densitometry. Representative blots were from three independent experiments. (e) Quantified western blot results of liver proteins. For the statistics of each panel in this figure, data are expressed as (). and versus the control group; and versus the model (CCl4) group.
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