Research Article
Withaferin A Exerts Preventive Effect on Liver Fibrosis through Oxidative Stress Inhibition in a Sirtuin 3-Dependent Manner
Table 1
The sequences of the primers for real-time PCR.
| | Gene | Forward primers | Reverse primers |
| | α-SMA | 5-CCACCATCTGCCTGAAATCC-3 | 5-GCTTCTTGTCCAGCCTCCTC-3 | | Fibronectin | 5-ACACGGTTTCCCATTACGCC-3 | 5-GGTCTTCCCATCGTCATAGCAC-3 | | α1(I) Procollagen | 5-GCTCCTCTTAGGGGCCACT-3 | 5-CCACGTCTCACCATTGGGG-3 | | SIRT1 | 5-AGTTCCAGCCGTCTCTGTGT-3 | 5-GATCCTTTGGATTCCTGCAA-3 | | SIRT2 | 5-TAGACACGCTGGAACGAGTG-3 | 5-TCTCTTTCATCCAGCCCATC-3 | | SIRT3 | 5-CAGCTACATGCACGGTCTGT-3 | 5-ACACAATGTCGGGTTTCACA-3 | | SIRT4 | 5-CATCCAGCACATTGATTTCG-3 | 5-CCAGTCTCTCCCAGTTGCTC-3 | | SIRT5 | 5-CCATCACCCAGAACATTGACG-3 | 5-ACAGTGCCACACGAGGTACA-3 | | SIRT6 | 5-GTGGATGAGGTGATGTGCAG-3 | 5-TCAGCCTTGAGTGCTACTGG-3 | | SIRT7 | 5-GACTGAGCGTACTGCCCTTC-3 | 5-TTCAGAGGCTGCCCTAATGT-3 | | GAPDH | 5-TGAACGGGAAGCTCACTGG-3 | 5-GCTTCACCACCTTCTTGATGTC-3 |
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