Protective Effect of Metformin against Hydrogen Peroxide-Induced Oxidative Damage in Human Retinal Pigment Epithelial (RPE) Cells by Enhancing Autophagy through Activation of AMPK Pathway

Figure 7

Protective effect of metformin in primary RPE cells. (a) Cells were treated with H₂O₂ (25-1600 μM) for 24 h, and cell viability was measured using the MTT assay. (b) Cells were pretreated with metformin at the indicated concentrations and then incubated with or without 200 μM H₂O₂ for further 24 h. (c) RPE cells were pretreated with 2.5 μM compound C for 30 min before 1 mM metformin treatment for 2 h and then incubated with or without 200 μM H₂O₂ for further 24 h. Cell viability was measured using the MTT assay. Data are presented as the ; , , and .

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