Protective Effect of Metformin against Hydrogen Peroxide-Induced Oxidative Damage in Human Retinal Pigment Epithelial (RPE) Cells by Enhancing Autophagy through Activation of AMPK Pathway
Table 2
shRNA sequence information.
Gene name
GenBank ID
Target sequence
Starting position
GC (%)
shAMPK1 (PRKAA2-RNAi1)
NM_006252
GGCTTATCATCTTATCATT
1028
31.58
shAMPK2 (PRKAA2-RNAi2)
NM_006252
TGTGAAAGAAGTGTGTGAA
938
36.84
shAMPK3 (PRKAA2-RNAi3)
NM_006252
CACTGAAACGAGCAACTAT
820
42.11
shBeclin1 (BECN1)
NM_003766
gaGAGGAGCCATTTATTGAAA
358
36.84
shLC3B
NM_022818
GTGCATGTCAGTTGTGGAGAA
1653
47.62
Note: sh-CTL control insertion sequence: TTCTCCGAACGTGTCACGT; shRNA structure: hU6-MCS-Ubiquitin-EGFP-IRES-puromycin.
