Curcumin-Induced DNA Demethylation in Human Gastric Cancer Cells Is Mediated by the DNA-Damage Response Pathway

Figure 1

Curcumin inhibits proliferation, colony formation, and migration of hGCCs. hGCCs were treated with different concentrations of curcumin for 24, 48, and 72 h, and the proliferation, colony formation, and migration of hGCCs were assayed. (a) The cell viability was measured by MTT assay and was calculated by the following formula: . (b) Cells were stained with crystal violet, the number of colonies was counted, and (c) the colony formation efficiency (%) was calculated: . (d) Cell migration was monitored by scratch wound healing assay. All experiments were carried out in triplicates, and the data are shown as the . The single factor variance analysis was used to compare the significance between groups: , , and . Difference with was considered statistically significant.

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