Curcumin-Induced DNA Demethylation in Human Gastric Cancer Cells Is Mediated by the DNA-Damage Response Pathway

Figure 3

DNA damage triggers DDR and downstream signaling pathways. hGCCs were treated with 20 μM curcumin for 24 h, and the expression of several genes in DDR or downstream signaling pathways was determined by RT-qPCR. (a) DNA damage-triggered DDR and downstream signaling pathways. (b) DDR-related gene expression. (c) Expression of p53 target genes. (d) Expression of E2F target genes. All experiments were carried out in triplicate, and the data are shown as the . The single factor variance analysis was used to compare the significance between groups as follows: , , and . Difference with was considered statistically significant.

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