Curcumin-Induced DNA Demethylation in Human Gastric Cancer Cells Is Mediated by the DNA-Damage Response Pathway

Table 1

Primers for RT-qPCR.

Primers	Sequence	Tm (°C)	Product length (bp)
ATM forward primer	5- GAGGAGCTTGGGCCTTTGTT -3	60.54	151
ATM reverse primer	5- TAAGTCACGGCTGTCTGGC -3	59.71
ATR forward primer	5- GGCAGTAGCTTCCTTTCGCT -3	60.39	165
ATR reverse primer	5- CTAATTGCACTGACTCCGGC -3	58.99
DNA-PK forward primer	5- GTGGCATCCAGAGTAGCGAA -3	59.82	165
DNA-PK reverse primer	5- TCGCAACCAGTTCACACAGA -3	59.82
γH2AX forward primer	5- CGACAACAAGAAGACGCGAAT -3	59.54	220
γH2AX reverse primer	5- CTCTTAGTACTCCTGGGAGGC -3	58.69
p21 forward primer	5- GACCATGTGGACCTGTCACT -3	59.31	176
p21 reverse primer	5- GCGGATTAGGGCTTCCTCTT -3	59.53
GADD45A forward primer	5- CCAAGGGGCTGAGTGAGTTC -3	60.32	153
GADD45A reverse primer	5- TCCTTCCTGCATGGTTCTTTGT -3	60.16
Cyclin E1 forward primer	5- GGGTATCAGTGGTGCGACAT -3	59.82	186
Cyclin E1 reverse primer	5- CATGGCTTTCTTTGCTCGGG -3	59.83
DNMT1 forward primer	5- GGCGGCTCAAAGATTTGGAA -3	59.11	161
DNMT1 reverse primer	5- CAGGTAGCCCTCCTCGGATA -3	59.89
HDAC1 forward primer	5- TGCTGTTAACTACCCGCTCC -3	59.75	187
HDAC1 reverse primer	5- ACACTTGGCGTGTCCTTTGA -3	60.11
GAPDH forward primer	5- CCATGGGGAAGGTGAAGGTC -3	60.03	172
GAPDH reverse primer	5- TGGAATTTGCCATGGGTGGA -3	59.88