Norepinephrine Induces Lung Microvascular Endothelial Cell Death by NADPH Oxidase-Dependent Activation of Caspase-3

Figure 5

Superoxide anion and hydrogen peroxide are involved in NE-induced RLMEC death. RLMEC were stimulated with NE (5 ng/mL) for 24 h. Detection of apoptotic cells was performed using (a) FragEL kit (Millipore) and by (b) DNA fragmentation. NE induced the appearance of RLMEC in late apoptotic stages as evidenced by both the FragEL and DNA fragmentation assays. These effects were inhibited in the presence pf PEG-SOD and PEG-CAT.

(a)

(b)