The Antiaging Activity of Ergothioneine in UVA-Irradiated Human Dermal Fibroblasts via the Inhibition of the AP-1 Pathway and the Activation of Nrf2-Mediated Antioxidant Genes

Figure 1

EGT suppressed MMP-1 and IL1β expression but enhanced the procollagen expression in HSF cells. (a) Chemical structure of ergothioneine (EGT). (b, c) Different concentrations of EGT (0.125-1 μM) or vehicle (PBS) were treated with HSF cells for 24 h. (b) The percentage of cell viability was measured by the MTT colorimetric method. The formula used to calculate the percentage of viable cells was . (c) EGT-mediated type I procollagen expression was measured by Western blot method. (d) HSF cells were pretreated with EGT (0.125-0.5 μM) for 24 h and then irradiated without or with 3 J/cm² UVA. The expression of MMP-1, IL1β proteins were measured by Western blot method against β-active as the internal control. Results were presented as of three or more assays. compared with untreated control cells.

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