Involvement of the miR-137-3p/CAPN-2 Interaction in Ischemia-Reperfusion-Induced Neuronal Apoptosis through Modulation of p35 Cleavage and Subsequent Caspase-8 Overactivation

Figure 1

Temporal changes in motor dysfunction and spinal CAPN subtype expression post IR. (a) Temporal changes in hind-limb motor function were evaluated using Tarlov’s scores at 0.5, 1, 4, 8, and 12 h after IR. per group. (b) Representative Western blots of CAPN-1 and CAPN-2 in injured spinal cord samples after IR. (c) Protein quantification of CAPN-1 and CAPN-2 levels after IR. The relative protein levels were normalized to those in the sham group. The data are expressed as the (SD). per group. versus the sham group. (d) Representative double immunofluorescence staining of CAPN-2 with spinal neurons (NeuN), microglia (Iba-1), and astrocytes (glial fibrillary acidic protein (GFAP)) in the anterior horns of spinal cords at 4 h after IR. The yellow labels with white arrows indicate colocalization. . per group. (e, f) Quantification of cells double-labeled with CAPN-2 immunoreactivity and markers of specific cell types. The data are presented as the average of three independent images of laminae II and III in the gray matter and expressed as the . versus the sham group.

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