Involvement of the miR-137-3p/CAPN-2 Interaction in Ischemia-Reperfusion-Induced Neuronal Apoptosis through Modulation of p35 Cleavage and Subsequent Caspase-8 Overactivation

Figure 2

IR induced aberrant spinal miR-137-3p expression and negatively regulated CAPN-2 expression in vivo. (a) A heat map representation of miRs differentially expressed in spinal cord samples at 4 h post IR. Three independent replicates were performed. The red signals indicate the upregulated miRs, and the green signals indicate the downregulated miRs. (b) Quantification of miR-137-3p expression post IR. per group. The data are expressed as the . versus the sham group. (c) The putative target binding site of miR-137-3p in the rat 3-UTR of CAPN-2 was predicted by the TargetScan database and confirmed by a luciferase reporter assay. versus the WT 3-UTR cells transfected with the miR-137-3p mimic. (d) Representative Western blots and protein quantification of CAPN-2 in the spinal cord after different treatments. β-Actin was used as a loading control. (e) Quantification of CAPN-2 mRNA expression after different treatments. (f) Hind-limb motor function was assessed by Tarlov’s scores after different treatments. versus the sham group; ^# versus the IR group.

(a)

(b)

(c)

(d)

(e)

(f)