Resveratrol Inhibits Ischemia-Induced Myocardial Senescence Signals and NLRP3 Inflammasome Activation

Figure 8

RSV inhibited the inflammasome in different cell types. (a, c, e) Representative blots of NLRP3 and caspase 1 p20 in NRCMs, CFs, and macrophages, respectively. (b, d, f) Relative expression levels of NLRP3 and caspase 1 p20 in NRCMs, CFs, and macrophages, respectively. (g, h) Flow cytometry detected immune cells (marked with CD45), macrophage (marked with F4/80), and NLRP3. (i, j, k) Quantitative analysis for CD45-positive cells, F4/80-positive cells, and double-positive (F4/80 and NLRP3) labeled cells, respectively (). Each experiment was repeated three times independently. Cells were harvested for western blot analysis after 12 h treatment. Data are presented as the . vs. the no treatment or RSV group, ^# the LPS+RSV group vs. LPS group.

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