Oxidized Oils and Oxidized Proteins Induce Apoptosis in Granulosa Cells by Increasing Oxidative Stress in Ovaries of Laying Hens

Table 1

Characteristics of the experimental oil and protein used.

Corn oil			Corn gluten meal
Item	Fresh oil	Oxidized oil	Item (g/kg)	Fresh protein	Oxidized protein
Acid value (g KOH/kg)	0.33	4.04	Dry matter	910.2	985.9
Peroxides (mEq O2/kg)	4.0	7.6	Crude protein	639.1	647.4
Fatty acid composition (g fatty acid/100 g total fatty acids)			Crude fat	50.3	31.0
16 : 0	12.57	15.39	Protein carbonyl (nmol/mg of protein)	7.95	40.09
18 : 0	1.67	2.07	Free sulfhydryl (nmol/mg of protein)	7.80	4.39
18 : 1	27.78	32.00	Total disulfide and sulfhydryl (nmol/mg of protein)	65.22	50.72
18 : 2 (n-6)	55.53	47.26
18 : 3 (n-3)	0.79	0.48