Amyloid Beta-Peptide Increases BACE1 Translation through the Phosphorylation of the Eukaryotic Initiation Factor-2α

Figure 2

Aβ_1-42 oligomers and H₂O₂ decrease cell viability. Human neuroblastoma cells were treated with increasing concentrations of (a) Aβ_1-42 oligomers or (d) H₂O₂ for 24 h. Cell survival was measured by MTT reduction. Data are of 4 independent experiments performed in triplicate. , vs. control by one-way ANOVA using Tukey’s posttest. Images of cells with the MTT reduced to blue formazan were taken after 24 h of treatment with (b) Aβ_1-42 oligomers or (e) H₂O₂. Bars represent 20 μm. The proapoptotic state of the cells was studied by the identification of cleavaged caspase-3 (green; marked with white arrows) after 24 h of treatment with (c) Aβ_1-42 oligomers or (f) H₂O₂. Nuclei were counterstained with Hoechst (pink). Bars represent 30 μm.

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