Oxidative Stress in Alzheimer’s Disease: In Vitro Therapeutic Effect of Amniotic Fluid Stem Cells Extracellular Vesicles

Figure 2

Effect of AFCS-EV supplementation on ROS modulation in AD primary neurons. (a) ROS and GSH content was measured with fluorescent probes, as described in Section 2.8, after 7 days of AFSC-EV exposure. value < 0.05; value < 0.01; value < 0.0001. (b) Western blot analysis of total lysate of WT, FAD cells treated or not with AFSC-EV, then revealed with the indicated primary antibodies. The graphs represent the of densitometric analysis of 3 experiments, normalized to actin values. value < 0.05; value < 0.01; value < 0.001. (c) Western blot analysis of AFSC-extracellular vesicles derived from 4 donors, then revealed for CD9, CD81, and Rab5, as exosome markers, SOD1, and actin. The graph represents the of densitometric analysis of 3 experiments, normalized to actin values. value < 0.05.

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