Oxidative Stress in Alzheimer’s Disease: In Vitro Therapeutic Effect of Amniotic Fluid Stem Cells Extracellular Vesicles

Figure 4

Effect of AFSC-EV supplementation on AD markers of AD primary neurons in vitro. (a) Western blot analysis of total lysate of WT and FAD cells treated or not with AFSC-EV, then revealed with anti-p-Tau^ser422 antibody. The graph represents the of densitometric analysis of 3 experiments, normalized to actin values. value < 0.001. (b) Representative images showing DAPI (blue) and p-Tau^ser422 (red) signals of FAD cells incubated or not with EV. . (c) Western blot analysis of total lysate of WT, FAD cells treated or not with AFSC-EV, then revealed with 6E10 antibody. APP (100 kDa) and Aβ oligo (16 kDa) bands, with 6E10, are shown. The graphs represent the mean ± SEM of densitometric analysis of 3 experiments, normalized to actin values. value < 0.05; value <0.01; value<0.001. (d) Representative images with DAPI (blue) and APP (green) signals of FAD cells incubated or not with AFSC-EV are shown. . (e) Percentage of secreted β-amyloid of FAD cells treated or not with AFSC-EV for 7 days. These data are obtained by measuring the Aβ42 levels via ELISA as described in Section 2.9. value < 0.001.

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