Anticancer Activity of a Novel High Phenolic Sorghum Bran in Human Colon Cancer Cells

Figure 2

Effect of high phenolic sorghum bran extract on apoptosis of multiple human colon cancer cell lines. (a–c) HCT15, SW480, and HCT116 cells were treated with 0, 1.25, and 2.5 mg/ml of high phenolic sorghum bran extract for 18 h, and apoptosis was measured using the Alexa Fluor 488 Annexin V/Dead Cell Apoptosis Kit. Values are (). (d–f) HCT15, SW480, and HCT116 cells were treated with 0, 1.25, and 2.5 mg/ml of high phenolic sorghum bran extract (HP) for 18 h, and western blot was performed to measure the expression of indicated proteins. Values are (). Significance is indicated by , , and (difference between vehicle and treatment); ^#, ^##, and ^### (difference between 1.25 mg/ml and 2.5 mg/ml).

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