Anticancer Activity of a Novel High Phenolic Sorghum Bran in Human Colon Cancer Cells

Figure 3

Effect of high phenolic sorghum bran extract on cell cycle distribution of multiple human colon cancer cell lines. (a–c) HCT15, SW480, and HCT116 cells were treated with 0, 1.25, and 2.5 mg/ml of high phenolic sorghum bran extract for 18 h, and the cell cycle distribution was measured by FACS analysis. Values are (). (d–f) HCT15, SW480, and HCT116 cells were treated with 0, 1.25, and 2.5 mg/ml of high phenolic sorghum bran extract (HP) for 18 h, and western blot was performed to measure the expression of indicated proteins. Values are (). Significance is indicated by , , and (difference between vehicle and treatment); ^#, ^##, and ^### (difference between 1.25 mg/ml and 2.5 mg/ml).

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