Gentiopicroside Ameliorates Oxidative Stress and Lipid Accumulation through Nuclear Factor Erythroid 2-Related Factor 2 Activation

Figure 2

Effects of FFA and GPS exposure on the PI3K/AKT signaling pathway, Nrf2 antioxidant pathway, and PPARα in HepG2 cells. (a, b) All cells were incubated with FFA for 0, 6, 12, 18, or 24 h. (c, d) These cells were subjected to diverse concentrations of GPS (0, 4, 20, 100, 200, and 500 μM) for 24 h. The levels of protein (PI3K/AKT, Nrf2, HO-1, NQO1, GCLM, and PPARα) were assessed by Western blot and quantified by ImageJ software. All data are presented as (three independent experiments). ^# and ^## vs. control group.

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