Gentiopicroside Ameliorates Oxidative Stress and Lipid Accumulation through Nuclear Factor Erythroid 2-Related Factor 2 Activation

Figure 7

Effects of GPS on the protein levels of AKT, Nrf2, HO-1, NQO1, GCLM, PPARα, and SREBP-1c in tyloxapol- (Ty-) treated mice. The Ty-induced mice were given by intraperitoneal injection with GPS (20, 40, or 80 mg/kg) or fenofibrate (100 mg/kg) for 1 h; after that, injected with Ty (500 mg/kg) for 12 h. (a) Western blot was used to analyze the levels of p-AKT, Nrf2, HO-1, NQO1, GCLM, PPARα, and SREBP-1c in the liver. (b–h) Quantification of p-AKT expression was standardized to AKT and of Nrf2, HO-1, NQO1, GCLM, PPARα, and SREBP-1c was standardized to β-actin, respectively. All data are presented as (, number of mice in each group). ^# and ^## vs. control group; and vs. Ty-stimulated group.

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