Ellagic Acid Protects Dopamine Neurons via Inhibition of NLRP3 Inflammasome Activation in Microglia

Figure 4

EA inhibited microglial NLRP3 inflammasome activation in vitro. BV-2 cells were treated with EA (0.1 and 1 μM) for 30 min and then incubated with LPS (100 ng/ml) for 24 h. Microglial activation was evaluated by immunostaining with an anti-OX-42 antibody (a) and quantitated by western blot analysis with an anti-Iba-1 antibody (b). . The effects of EA on NLRP3 inflammasome signaling activation in BV-2 cells were detected via western blotting (b). The ratios of densitometry values of Iba-1, NLRP3, caspase-1, and pro-caspase-1 with β-actin were analyzed and normalized to each respective control cultures. The release of proinflammatory factors, such as TNF-α, IL-1β, and IL-18, in BV-2 cell culture medium was measured by ELISA (c). Data were the from three independent experiments performed in triplicate. compared with control cultures; ^# compared with LPS-treated cultures.

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