Research Article
3-Amino-1,2,4-Triazole Induces Quick and Strong Fat Loss in Mice with High Fat-Induced Metabolic Syndrome
Table 1
Primer sequences used for real-time quantitative PCR (amplicons between 100 and 150 bp).
| | Primers | Sequence forward and reverse (5–3) |
| | Adiponectin | F: GGAACTTGTGCAGGTTGGAT | | R: CCTTCAGCTCCTGTCATTCC | | Adrβ3 | F: GCTGACTTGGTAGTGGGACTC | | R: TAGAAGGAGACGGAGGAGGAG | | Caspase 1 | F: ACCCTCAAGTTTTGCCCTTT | | R: GATCCTCCAGCAGCAACTTC | | Catalase | F: CACGCTGGTAGTTGGCCACT | | R: GCCCAGCCCTGACAAAATGC | | CCL2 | F: GCCAACTCTCACTGAAGCC | | R: GCTGGTGAATGAGTAGCAGC | | CD68 | F: TAGGACCGCTTATAGCCCAAG | | R: CTGTAGGTGTCATCGTGAAG | | CIDEA | F: GCAGCCTGCAGGAACTTATC | | R: CCGATTTCTTTGGTTGCTTG | | GAPDH | F: CCATCACCATCTTCCAGGAG | | R: GTGGTTCACACCCATCACAA | | GLUT4 | F: TGATTCTGCTGCCCTTCTGT | | R: GGACATTGGACGCTCTCTCT | | HSL | F: ACGGATACCGTAGTTTGGTGC | | R: TCCAGAAGTGCACATCCAGGT | | IR | F: CCACCAATACGTCATTCACAAC | | R: GGGCAGATGTCACAGAATCAA | | Interleukin-1β | F: GCCACCTTTTGACAGTGATGAG | | R: CCTGAAGCTCTTGTTGATGTGC | | Interleukin 18 | F: TCTGACATGGCAGCCATTGT | | R: CAGGCCTGACATCTTCTGCAA | | Lep | F: CGTGTGTGAAATGTCATTGATCCT | | R: GACACCAAAACCCTCATCAAGAC | | LPL | F: AGTGGCCGAGAGCGAGAAC | | R: CCACCTCCGTGTAAATCAAGAAG | | PPARγ | F: TCAGCTCTGTGGACCTCTCC | | R: ACCCTTGCATCCTTCACAAG | | RevErbα | F: TGGCCTCAGGCTTCCACTATG | | R: CCGTTGCTTCTCTCTCTTGGG | | SOD1 | F: GACGGTGTGGCCAATGTGTC | | R: CAAGCGGCTCCCAGCATTTC | | TNF-α | F: GTCTACTGAACTTCGGGGTGA | | R: CTCCTCCACTTGGTGGTTTG | | UCP2 | F: GCATTGGCCTCTACGACTCT | | R: GTCCTGGTATCTCCGACCAC |
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Adrβ3: adrenergic β3 receptor; CCL2 or MCP1: monocyte chemoattractant protein 1; CD68: cluster of differentiation 68; CIDEA: cell death activator; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; GLUT4: glucose transporter type 4; HSL: hormone-sensitive lipase; IR: insulin receptor; Lep: leptin; LPL: lipoprotein lipase; PPARγ: peroxisome proliferator-activated receptor gamma; RevErbα or NR1D1: nuclear receptor subfamily 1; SOD1: superoxide dismutase 1; TNF-α: tumor necrosis factor alpha; UCP2: uncoupling protein 2.
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