lncRNA OTUD6B-AS1 Exacerbates As₂O₃-Induced Oxidative Damage in Bladder Cancer via miR-6734-5p-Mediated Functional Inhibition of IDH2

Figure 6

IDH2 knockdown exacerbates miR-6734-5p-induced oxidative damage. (a–d) ROS, MDA, SOD, and GSH-Px production in bladder cancer cells in response to IDH2 knockdown or transfection of mimics. (e–g) Nrf2 expression in cytoplasm and nucleus of bladder cancer cells in response to IDH2 knockdown or transfection of mimics. (h) Apoptosis was determined by flow cytometry under IDH2 knockdown or transfection of mimics. (i) Statistical analysis of apoptosis rates determined by flow cytometry. (j) Apoptosis was determined by TUNEL staining following IDH2 knockdown or transfection of mimics. (k) Statistical analysis of cells positive for TUNEL staining. (l) Cell viability determined by CCK-8 assay under IDH2 knockdown or transfection of mimics. , vs. control cells; ^#, ^## vs. cells with IDH2 knockdown; ^&, ^&& vs. cells transfected with mimics.

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