Neochamaejasmin A Induces Mitochondrial-Mediated Apoptosis in Human Hepatoma Cells via ROS-Dependent Activation of the ERK1/2/JNK Signaling Pathway

Figure 6

Effect of NCA on JNK/ERK signaling molecules in HepG2 cells. (a, b) HepG2 cells were treated with NCA for 48 h, and the levels of JNK, p-JNK, ERK1/2, and p-ERK1/2 were examined by Western blot. (c, d) HepG2 cells were pretreated with 10 μM SP600125 for 1 h and subsequently treated with 73.7 μM NCA for 48 h, and the levels of JNK and p-JNK were then examined by Western blot. (e, f) HepG2 cells were pretreated with 10 μM PD0325901 for 1 h and subsequently treated with 73.7 μM NCA for 48 h, and then the levels of ERK1/2 and p-ERK1/2 were examined by Western blot. and , compared with the control group.

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