Salidroside Ameliorates Mitochondria-Dependent Neuronal Apoptosis after Spinal Cord Ischemia-Reperfusion Injury Partially through Inhibiting Oxidative Stress and Promoting Mitophagy

Figure 2

Sal inhibited intrinsic apoptosis of spinal cord neurons after SCIRI in vivo.(a, A) Representative images of NeuN, TUNEL, and DAPI costaining of spinal cord sections of mice that were subjected to sham operation or SCIRI for 12 h. Scale bar, 100 μm. (a, B and C) Immunostaining of activated caspase-9 (B; in green) and activated caspase-3 (C; in green) in the spinal cords of mice. Representative images were colabeled with NeuN (in red) and DAPI. Scale bar, 100 μm. Quantification of (b) TUNEL-positive cells and (c, d) cleaved caspase-3/caspase-9 intensity normalized to their respective levels in sham-operated mice. (e) Representative western blots of cleaved caspase-3/caspase-9, Bcl-2, and Bax expression in the spinal cords of mice that were subjected to sham operation or SCIRI for 12 h. (f) Quantification of activated caspase-3/caspase-9 and the ratio of Bcl-2 to Bax normalized to the levels in sham-operated mice on the basis of the western blots previously described. β-Actin was used as loading control. All values in this figure were presented as (); , , and .

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