Salidroside Ameliorates Mitochondria-Dependent Neuronal Apoptosis after Spinal Cord Ischemia-Reperfusion Injury Partially through Inhibiting Oxidative Stress and Promoting Mitophagy

Figure 7

Mdivi-1, a specific mitophagy inhibitor, partially reversed the protective effect of Sal on spinal cord neurons. (a) Representative images of TUNEL and DAPI costaining of spinal cord neurons that were subjected to control or OGD/R for 12 h. Scale bar, 100 μm. (b) Quantification of apoptotic (TUNEL+) cells was performed. (c) Representative western blots of cleaved caspase-3/caspase-9, Bcl-2, and Bax expression in neurons that were subjected to control or OGD/R for 12 h. (d) Quantification of activated caspase-3/caspase-9 expression and the ratio of Bcl-2 to Bax normalized to the level of neurons in the control group that were pretreated with DMSO. GAPDH was used as a loading control. (e) Representative images show ROS production in spinal cord neurons and quantification of ROS intensity normalized to the level in control neurons. Scale bar, 100 μm. Values are expressed as the ratio of fluorescence intensity relative to the control. All values are expressed as (); , , and .

(a)

(b)

(c)

(d)

(e)