The Radioprotective Effect of Procaine and Procaine-Derived Product Gerovital H3 in Lymphocytes from Young and Aged Individuals

Figure 4

In vitro inhibitory effect of procaine and Gerovital H3 (GH3) on lipid peroxidation in Jurkat cells (a–c), human serum (d), rat liver mitochondria (e), and human macrophage-induced LDL oxidation (f). Jurkat cells were preincubated with 0 (control), 2.5 (a), 5.0 (b), or 10 mM (c) of procaine hydrochloride equivalents for the indicated time points. Lipid peroxidation was induced by cumene hydroperoxide, and curcumin served as a control for inhibitory effect on lipid peroxidation. Human serum (d) and rat liver mitochondria (e) samples were pretreated with 0 (control), 0.5, 1.0, 2.0, 5.0, or 10 mM of procaine hydrochloride equivalents. Human macrophage-induced LDL oxidation (f) was assessed in human U937 monocyte-like cell line. Cells were treated with 0 (control), 0.5, 1.0, or 2.0 mM of procaine hydrochloride equivalents, and TBARS was quantified. Statistical analyses for (a–c) and (f) were performed using two-way ordinary ANOVA and Sidak’s multiple comparisons test. Statistical analyses for (d, e) were performed using two-way RM ANOVA and Sidak’s multiple comparisons test. Error bars mean standard deviations of 3 or 4 experiments. Statistical significance between GH3 and procaine: ; .

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