Novel Fluorescent Mitochondria-Targeted Probe MitoCLox Reports Lipid Peroxidation in Response to Oxidative Stress In Vivo

Figure 5

The relationship between lipid peroxidation, as measured by MitoCLox, and decrease in mitochondrial membrane potential under oxidative stress induced by H₂O₂. MRC5-SV40 cells were incubated with MitoCLox (200 nM) for 1 h before addition of H₂O₂ (0.5 mM). TMRM was added for 15 min before measurements to estimate the membrane potential. Flow cytometry analyses were performed using a Beckman Coulter FC 500. All experiments were triplicated, and each bar represents the .