Thioridazine Induces Cardiotoxicity via Reactive Oxygen Species-Mediated hERG Channel Deficiency and L-Type Calcium Channel Activation

Figure 1

THIO treatment reduces hERG protein levels and hERG current. (a) Western blot results and statistics for hERG expression in the absence or presence of THIO (incubation for 24 h). THIO significantly reduced 155 kDa of the hERG channel in a concentration-dependent manner (0.1, 1, and 3 μM) (). (b) Immunofluorescence showed reduced hERG protein expression by incubation with 3 μM THIO. Scale bar, 50 μm. (c) Protocol and examples on the hERG current under control or THIO-treated conditions. (d) - curve of the hERG current. THIO concentration dependently reduced the hERG current (). vs. control.

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