Thioridazine Induces Cardiotoxicity via Reactive Oxygen Species-Mediated hERG Channel Deficiency and L-Type Calcium Channel Activation

Figure 8

THIO induces prolongation of APD in mouse hearts and hiPS-CMs. (a, b) Action potential duration at 50% repolarization (APD₅₀) and 90% repolarization (APD₉₀) maps is shown from a heart under control conditions (left) and after THIO application (right). (c) Representative optical action potential traces under control (black line) and THIO (red line) conditions. (d) Representative examples of EADs in mouse hearts after THIO application. (e) Statistics of APD₅₀. (f) Statistics of APD₉₀. . (g) Representative current clamp recordings of action potentials under control conditions (black line) and 3 μM THIO (red line) and 3 μM THIO+3 mM NAC (green lines) treatment for 24 h in hiPS-CMs. (h) Statistics of APD₅₀ and APD₉₀. -8. vs. control and vs. THIO.

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