SOCS2 Inhibits Mitochondrial Fatty Acid Oxidation via Suppressing LepR/JAK2/AMPK Signaling Pathway in Mouse Adipocytes

Figure 2

SOCS2 reversed the promoting function of leptin on fatty acid oxidation. Eight-week-old male mice were intraperitoneally injected with leptin (1 mg/kg body weight) or saline. (a) SOCS2 mRNA level in iWAT after Ad-SOCS2 or sh-SOCS2 injection and leptin injection into mice (). Serum FFA (b) and TG (c) levels of mice after Ad-SOCS2 or sh-SOCS2 injection and leptin injection (). (d) Palmitate incorporation into iWAT after Ad-SOCS2 or sh-SOCS2 injection and leptin injection (). (e) Relative palmitate oxidation to CO₂ after Ad-SOCS2 or sh-SOCS2 injection and leptin injection in iWAT (). Protein expression levels of FABP4 (f), CPT-1b (g), and p-ACC (h) in iWAT after Ad-SOCS2 or sh-SOCS2 injection and leptin injection (). (i) Leptin receptor mRNA level in iWAT after Ad-SOCS2 or sh-SOCS2 injection and leptin injection (). All the protein levels (f–h) were detected by the ELISA test. Values are the . , .

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