Oxidative Medicine and Cellular Longevity / 2020 / Article / Fig 4

Research Article

SOCS2 Inhibits Mitochondrial Fatty Acid Oxidation via Suppressing LepR/JAK2/AMPK Signaling Pathway in Mouse Adipocytes

Figure 4

SOCS2 reduced mitochondrial respiratory activity. (a) mRNA expressions of the mitochondrial function genes after transfection with Ad-SOCS2 and sh-SOCS2 for 48 h (). (b, c) The activities of mitochondrial complexes I and III after vector delivery and differentiation for 8 days (). (d) Images of adipocytes Cyt C immunofluorescent staining after vector delivery and differentiation for 8 days (red). Scale bar: 100 μm (). (e) ATP level after vector delivery and differentiation for 8 days (). (f) mtDNA copy number after vector delivery and differentiation for 8 days (). All the protein levels (b, c) were detected by the ELISA test. Values are the . , .
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