Oxidative Medicine and Cellular Longevity / 2020 / Article / Fig 5

Research Article

SOCS2 Inhibits Mitochondrial Fatty Acid Oxidation via Suppressing LepR/JAK2/AMPK Signaling Pathway in Mouse Adipocytes

Figure 5

SOCS2 inhibited leptin-induced fatty acid oxidation in mouse adipocytes. Preadipocytes from mice iWAT were transfected with control vector, Ad-SOCS2, and sh-SOCS2 induced differentiation for 8 days, incubated with 100 nm leptin for 24 h on the last day before being harvested. (a) mRNA level of leptin receptor after transfection and leptin treatment (). (b) Cellular FFA content after transfection and leptin treatment (). (c) Fatty acid oxidation. Palmitate oxidation to CO2 was measured for 3 h after transfection and leptin treatment (). (d–h) p-ACC, CPT-1b, FABP4, PGC-1α, and Cyt C protein levels after transfection and leptin treatment (). All the protein levels were detected by the ELISA test. (i) ATP level after transfection and leptin treatment (). Values are the . , .
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