SOCS2 Inhibits Mitochondrial Fatty Acid Oxidation via Suppressing LepR/JAK2/AMPK Signaling Pathway in Mouse Adipocytes

Figure 8

SOCS2 reduced mitochondrial fatty acid oxidation through inhibiting the JAK2/AMPK pathway. (a) Protein levels of p-JAK2^Tyr1007-1008, JAK2, p-AMPK^Thr172, and AMPK with Ad-SOCS2 or sh-SOCS2 transfection for 48 h and 1 nM AZD1480 (JAK2 inhibitor) incubation for 2 h (). (b) Protein expression levels of CPT-1b, p-ACC, ACC1, and PGC-1α with Ad-SOCS2 or sh-SOCS2 transfection for 48 h and 1 nM AZD1480 incubation for 2 h (). (c) Protein expression levels of p-JAK2^Tyr1007-1008, JAK2, p-AMPK^Thr172, and AMPK with Ad-SOCS2 or sh-SOCS2 transfection for 48 h and 1 nM Compound C (AMPK inhibitor) incubation for 1 h (). (d) Protein expression levels of CPT-1b, p-ACC, ACC1, and PGC-1α with Ad-SOCS2 or sh-SOCS2 transfection for 48 h and 1 nM Compound C incubation for 1 h (). GAPDH was used as the loading control in western blot. Values are the . , .

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