Stimulation of Epithelial Sodium Channels in Endothelial Cells by Bone Morphogenetic Protein-4 Contributes to Salt-Sensitive Hypertension in Rats

Figure 1

HS diet elevates blood pressure in a time-dependent manner, reduces endothelium-dependent artery relaxation, and stimulates endothelial ENaC in SS rats. SBP (a), DBP (b), and MAP (c) were measured in SD and SS rats fed NS (0.3%) or HS (5%) diet for 0, 7, 14, and 21 days. represents vs. SS-NS, SS rats fed NS ( rats for each group). (d, e) Representative traces of ACh-induced artery relaxation in NS or HS diet-fed SD and SS rats and summary of relaxation responses of mesenteric arteries to different doses of ACh under the conditions shown in (d). The first dot in each plot represents the time when Phe (10 μM) was added. The following dots indicate ACh concentrations gradually increased to 0.1 nM, 1 nM, 10 nM, 100 nM, 1 μM, 10 μM, and 100 μM. indicates vs. SS-NS ( for each group). (f) Summary of relaxation responses of mesenteric arteries from these rats to different doses of NTG ( for each group). (g) Representative ENaC single-channel currents generated by split-open artery technique in intact endothelial cells from NS or HS diet-fed SD and SS rats, under the indicated conditions. (h) Summary of calculated ENaC obtained from single-channel recordings from the different experimental groups as shown in (g). represents vs. SS-NS ( rats for each group).

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