Stimulation of Epithelial Sodium Channels in Endothelial Cells by Bone Morphogenetic Protein-4 Contributes to Salt-Sensitive Hypertension in Rats

Figure 5

HS activates Sgk1/Nedd4-2 via BMP4 and p38 MAPK signaling. (a) Representative Western blots and summarized data showing BMP4 protein levels in endothelial cells isolated from SS rats either under control conditions or treated with an additional 40 mM NaCl in the culture medium for 24 hours. The data are the of 6 experiments in each group. indicates vs. control. (b) Representative Western blots and summarized data showing p38 MAPK and p-p38 MAPK protein levels under control conditions or treated with HS additional 40 mM NaCl in the culture medium for 24 hours, in the absence of and in the presence of 100 ng/mL noggin. indicates vs. control; ^#represents vs. HS ( for each group). (c–d) Representative Western blots and summarized data showing Sgk1, p-Sgk1, Nedd4-2, and p-Nedd4-2 protein levels under control conditions or treated with additional 40 mM NaCl in the culture medium for 24 hours, in the absence of and in the presence of either 100 ng/mL noggin or 10 μM SB202190 for 24 hours. indicates vs. control; ^#represents vs. HS ( for each group). (e–h) Representative Western blots and summarized data showing BMP4, p-p38 MAPK, p38 MAPK, p-Sgk1, Sgk1, p-Nedd4-2, and Nedd4-2 protein levels in primary cultured endothelial cells isolated from SS rats either under control conditions or treated with an additional 80 mM mannitol in the culture medium for 24 hours. The data are the of 6 experiments in each group.

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