Effects of PTE on the SH-SY5Y and BV-2 coculture systems. The BV-2 cells were incubated with vehicle control or PTE (2.5, 5.0, or 10.0 μM) for 2 h, followed by cocultivation with SH-SY5Y cells for 24 h. (a) The morphology of SH-SY5Y cells was photographed under an inverted/phase-contrast microscope, and (b) viability was quantified using a CCK-8 assay. (c) The LDH release in supernatants was determined using an LDH release assay and expressed as the percentage of maximum. (d) The expression of SIRT-1 in BV-2 cells was measured using western blot analysis and normalized to that of β-actin. Data are shown as ..α, compared with the control. β, compared with PTE 2.5 μM.
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