Cerebral Mitochondrial Function and Cognitive Performance during Aging: A Longitudinal Study in NMRI Mice

Table 1

Oligonucleotide primer sequences, product sizes, and primer concentrations for quantitative real-time PCR.

Primer	Sequence	Product size (bp)	Conc. (μM)
AMPK (β-subunit)	5-agtatcacggtggttgctgt-3; 5-caaatactgtgcctgcctct-3	190	0.1
B2M	5-ggcctgtatgctatccagaa-3; 5-gaaagaccagtccttgctga-3	198	0.4
BDNF	5-gatgccagttgctttgtctt-3; 5-atgtgagaagttcggctttg-3	137	0.1
CI (NADH-ubiquinone oxidoreductase 51 kDa subunit)	5-acctgtaaggaccgagaga-3; 5-gcaccacaaacacatcaaaa-3	227	0.1
CIV (cytochrome c oxidase subunit 5A)	5-ctgttccattcgctgctatt-3; 5-gcgaacagcactagcaaaat-3	217	0.1
Creb-1	5-tagctgtgacttggcattca-3; 5-ttgttctgtttgggacctgt-3	184	0.5
CS	5-aacaagccagacattgatgc-3; 5-atgaggtcctgctttgtcct-3	184	0.1
GAP43	5-agggagatggctctgctact-3; 5-gaggacggggagttatcagt-3	190	0.15
Nrf-1	5-tcggagcacttactggagtc-3; 5-ctagaaaacgctgccatgat-3	228	0.5
PGC1-α	5-tgtcaccaccgaaatcct-3; 5-cctggggaccttgatctt-3	124	0.05
PGK1	5-gcagattgtttggaatggtc-3; 5-tgctcacatggctgacttta-3	185	0.4
SOD2	5-acagcgcatactctgtgtga-3; 5-gggggaacaactcaactttt-3	183	0.1
SYP1	5-tttgtggttgttgagttcct-3; 5-gcatttcctccccaaagtat-3	204	0.1
Tfam	5-agccaggtccagctcactaa-3; 5-aaacccaagaaagcatgtgg-3	166	0.5

bp: base pairs; conc.: concentration.