Oxidative Medicine and Cellular Longevity / 2020 / Article / Fig 5

Research Article

Short-Chain Fatty Acids Ameliorate Diabetic Nephropathy via GPR43-Mediated Inhibition of Oxidative Stress and NF-κB Signaling

Figure 5

SCFA-mediated antioxidant and anti-inflammatory effects were partly reversed by siRNA-GPR43. qRT-PCR was performed to detect GPR43 and GPR41 mRNA levels. (b) Western blotting-based assay for the expression of GPR43 and MCP-1 after a 30 mM high glucose challenge for 6, 12, and 24 h. (c) The effects of indicated concentrations of SCFAs or a GPR43 agonist on GPR43 and MCP-1 expression were analyzed by western blotting. Following 30 mM high glucose for 24 h, SCFAs or a GPR43 agonist-ameliorated ROS (d), MDA (e), NF-κB signal (g), and MCP-1 (f) were significantly reversed by siRNA-GPR43. Ac: acetate; Pr: propionate; But: butyrate; compared with the NC group; # compared with the HG group. & compared with the Ac or Pr group.

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