Anti-Diabetic Atherosclerosis by Inhibiting High Glucose-Induced Vascular Smooth Muscle Cell Proliferation via Pin1/BRD4 Pathway

Figure 2

Effect of juglone and JQ1 treatment on thoracic aortic plaques in diabetic Apoe-/- mice. (a). HE staining of the mouse thoracic aorta (scale bars =100 μm). In the C57BL/6 group, no plaques were found in the intima of the thoracic aorta, the intima was smooth and flat, and the thickness of the middle membrane was uniform. The Apoe-/- group showed lipid deposition, a rough intima, and an irregular media. In the DM group, large plaques were found, the inner membrane was rough and discontinuous, and cells at the bottom of plaques were prominently proliferated and irregular. There was only lipid deposition in the intima of the thoracic aorta in the juglone group. The intima was smoother in the juglone group than in the DM group, with a small amount of hyperplasia at the lipid base. Only small plaques were found in the thoracic aorta lumen in the JQ1 group. Additionally, cells at the top of plaques proliferated to form fibrous caps, and there was only a small amount of cell proliferation at the bottom of plaques. (b). Analysis of the plaque area in HE-stained thoracic aorta of mice. Values are mean ± SEM, with n =10 mice per group. vs. C57BL/6; ^#P <0.05 vs. Apoe-/-;^&P <0.05 vs. STZ-Apoe-/-.

(a)

(b)