Anti-Diabetic Atherosclerosis by Inhibiting High Glucose-Induced Vascular Smooth Muscle Cell Proliferation via Pin1/BRD4 Pathway

Figure 6

Effects on Pin1, BRD4, cyclin D1, and MMP-9 protein expression levels, and proliferation and migration of VSMCs induced by high glucose when Pin1 is overexpressed. (a) Effects on Pin1, BRD4, cyclin D1, and MMP-9 protein expression levels in VSMCs induced by high glucose when Pin1 is overexpressed. Pin1 plasmid vector was transduced to VSMCs by the liposome method and treated with or without high glucose (25 mM) for 48 h. Cell protein was extracted for western blotting. (b-e) Relative ratios of Pin1, BRD4, cyclin D1, and MMP-9 over β-actin were determined by densitometric analysis. (f) Effects on proliferation of VSMCs induced by high glucose when Pin1 is overexpressed. The proliferation ability of cells was determined by the MTT assay (the experimental process was as described above). (g, h) Effects on migration of VSMCs induced by high glucose when Pin1 is overexpressed. The migration ability of cells was determined by the transwell assay (the experimental process was as described above) (scale bars =50 μm). Values are mean ± SEM (, 6 experiments per group). vs. Control; vs. HG (25 mM); vs. HG (25 mM) + juglone 10^-5 M.

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