Research Article
Nitrative Stress-Related Autophagic Insufficiency Participates in Hyperhomocysteinemia-Induced Renal Aging
Figure 2
Autophagic insufficiency was involved in HHcy-induced renal aging. (a) Representative Western blot showing the protein expression levels of autophagy in HHcy. Graphs representing (b, c) LC3 by quantitative analysis. (, , -5). (d) Graph representing quantitative analysis showing the mRNA level of LC3 in HHcy (, , and ; HHcy vs. Control; -6). (e) Representative Western blot showing the protein expression levels of aging and autophagy in MPC-5 treated with rapamycin. Graphs representing (f) p53, (g) p16, and (h) LC3 by quantitative analysis (, , and , Hcy vs. Control, rapamycin vs. Hcy, -4). (i) Representative Western blot showing the protein expression levels of aging and autophagy in MPC-5 infected with Atg5 adenovirus. Graphs representing (j) p53, (k) p21, and (l) LC3 by quantitative analysis (, , and , Hcy vs. Control, Atg5 adenovirus vs. Hcy, -4). Control: control group; Hcy: homocysteinemia group; Ad-Atg5: Atg5 adenovirus. Data were expressed as .
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